Multichannel analytics development

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(Created to document project progress as-it-happens and encourage people to actually touch the wiki)
 
(August 2011)
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==August 2011==
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=August 2011=
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== Image Preprocessing Improvements ==
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*Background subtraction used to remove the blur and imaging artifacts
 +
 
 +
== Tiling Functions for Microglia Tracing and Nuclei Segmentation: ==
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*Montage can be split into smaller tiles for processing.
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*Workflow of individual tiles can be distributed.
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**Tracing Tiles of Microglia
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**Segment and Classify Nuclei
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*Resulting Files can be stitched back into Global Coordinates
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== Soma Detection and Segmentation Improvements ==
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*Old method of morphological functions on the GFP channel missed somas.
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*Correlate Nuclei Channel to Microglia channel to identify somas

Revision as of 20:06, 30 August 2011

Contents

August 2011

Image Preprocessing Improvements

  • Background subtraction used to remove the blur and imaging artifacts

Tiling Functions for Microglia Tracing and Nuclei Segmentation:

  • Montage can be split into smaller tiles for processing.
  • Workflow of individual tiles can be distributed.
    • Tracing Tiles of Microglia
    • Segment and Classify Nuclei
  • Resulting Files can be stitched back into Global Coordinates

Soma Detection and Segmentation Improvements

  • Old method of morphological functions on the GFP channel missed somas.
  • Correlate Nuclei Channel to Microglia channel to identify somas
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