Multichannel analytics development
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+ | == Image Preprocessing Improvements == | ||
+ | |||
+ | *Background subtraction used to remove the blur and imaging artifacts | ||
+ | |||
+ | == Tiling Functions for Microglia Tracing and Nuclei Segmentation: == | ||
+ | |||
+ | *Montage can be split into smaller tiles for processing. | ||
+ | *Workflow of individual tiles can be distributed. | ||
+ | **Tracing Tiles of Microglia | ||
+ | **Segment and Classify Nuclei | ||
+ | *Resulting Files can be stitched back into Global Coordinates | ||
+ | |||
+ | == Soma Detection and Segmentation Improvements == | ||
+ | |||
+ | *Old method of morphological functions on the GFP channel missed somas. | ||
+ | *Correlate Nuclei Channel to Microglia channel to identify somas |
Revision as of 20:06, 30 August 2011
Contents |
August 2011
Image Preprocessing Improvements
- Background subtraction used to remove the blur and imaging artifacts
Tiling Functions for Microglia Tracing and Nuclei Segmentation:
- Montage can be split into smaller tiles for processing.
- Workflow of individual tiles can be distributed.
- Tracing Tiles of Microglia
- Segment and Classify Nuclei
- Resulting Files can be stitched back into Global Coordinates
Soma Detection and Segmentation Improvements
- Old method of morphological functions on the GFP channel missed somas.
- Correlate Nuclei Channel to Microglia channel to identify somas