Multichannel analytics development
From FarsightWiki
Contents |
August 2011
Image Preprocessing Improvements
- Background subtraction used to remove the blur and imaging artifacts
Tiling Functions for Microglia Tracing and Nuclei Segmentation:
- Montage can be split into smaller tiles for processing.
- Workflow of individual tiles can be distributed.
- Tracing Tiles of Microglia
- Segment and Classify Nuclei
- Resulting Files can be stitched back into Global Coordinates
Soma Detection and Segmentation Improvements
- Old method of morphological functions on the GFP channel missed somas.
- Correlate Nuclei Channel to Microglia channel to identify somas