August 2011

Image Preprocessing Improvements

• Background subtraction used to remove the blur and imaging artifacts

Tiling Functions for Microglia Tracing and Nuclei Segmentation:

• Montage can be split into smaller tiles for processing.
• Workflow of individual tiles can be distributed.
  • Tracing Tiles of Microglia
  • Segment and Classify Nuclei
• Resulting Files can be stitched back into Global Coordinates

Soma Detection and Segmentation Improvements

• Old method of morphological functions on the GFP channel missed somas.
• Correlate Nuclei Channel to Microglia channel to identify somas