Getting there

A fully automatic nuclear segmentation result has been generated, but it is not quite acceptable. This page describes the steps to take to move from "automated result" to "acceptable result"

• 

  Step 1. "Find Tiny Islands" Sort the table by volume and eliminate all objects with a shared boundary = 0. This is best done by selecting these objects in the table view (while holding the Ctrl key). A group delete function may be used to remove these objects. In this step you may also explore other tiny objects with a very small, but non-zero, shared boundary.

• 

  Step 2. "Find Huge Objects" Using the table or the scatter plot, inspect and edit very large objects(in volume and/or surface area - usually SPLIT)

• 

  Step 3. "Find Fragments" Using a scatter plot of volume vs. shared boundary, inspect and edit objects that have a small volume and large shared boundary. It is most likely that these objects will need to be MERGED.

• 

  Step 4. "Verify" Scan Image or randomly select objects to verify that the result is acceptable and can now be used for quantitative study.