Worm Features

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Features

Worms are tracked according to their distance from the pheromone spots and intrinsic features are computed from the image data for each worm.

Name

Description

Formula

Width

Model of worm Width

\overline{w}\times(1-e^{-0.1\times(m-|2i-m|)})

Area

Number of pixels in the worm

| Ω |

Minor Length

Minor axis length of an ellipse with the same area as the worm which has been fit to our worm model

4\sqrt{\lambda_0}

Major Length

Major axis length of an ellipse with the same area as the worm which has been fit to our worm model

4\sqrt{\lambda_1}

Eccentricity

Ratio of maximum to minimum distance of the center of mass from the worm’s surface

\cfrac{\max(\sqrt{||p-\overline{p}||})}{\min(\sqrt{||p-\overline{p}||})}

Orientation

Angle between the first principal axis of a given worm and the ‘x’ axis

\arctan[\cfrac{dy}{dx}]

t_start

Frame number that worm was initially detected

Ft(0)

t_end

Frame number that worm was last detected

F_t(\infty)

t_length

Total number of frames worm was tracked in a given image sequence

\sum_t F_t, F = \# of frames

Curvature

A metric to give an estimate of coiling as a worm behavior

\kappa = (\cfrac{\theta_i}{L})

Sum Squared Curvature

Average Curvature squared, gives an estimate of the worm’s bending energy

\sum_{i,t} mean(\cfrac{\theta_i^t}{L})

Intensity

The average intensity of the pixels in a worm

mean( | Ω | )

Worm_Velocity

The average worm speed

mean([ui,...,un]) i=1,2,...,15

Peri_std

The standard deviation of the worm speed. Gives users insight into irregularities of worm peristaltic progression

std([ui,...,un]) i=1,2,...,15

D_head_std

Standard deviation of the displacement of the worm’s head

std(\gamma_k^H= {\tilde{\gamma}}^H \times e^{-0.09 \times (m-k)})

D_tail_std

Standard deviation of the displacement of the worm’s tail

std(\gamma_k^T= {\tilde{\gamma}}^T \times e^{-0.72 \times (k)})

Reversal Rate

The frequency of backward progression by the worm’s head

\cfrac{||\sum u_i||}{\sum ||u_i||}

Class

Each Phenotype is given a separate class from 1-N

N / A

Bearing

The angle between the velocity vector and the spatial vector from the worm’s position to the center of the pheromone spot(chemical gradient)

\theta = \arctan[\cfrac{dv}{ds}]

Instantaneous Turning Rate

The change in orientation over time

[\cfrac{d\phi}{dt}]